Subtract Background

Main Menu: Image > Adjust > Subtract Background

Subtracting a Background Image provides for correction of non-uniform field defects in acquired images by subtracting a flat field from an acquired image.  To use this function, you should first acquire a flat field by grabbing a picture from your camera or microscope without a specimen in the field.  Then, grab an image with a specimen in it.

The Subtract Background command will produce a resulting image that has the background subtracted from it.

Only grayscale images are supported.

Given the finite range of gray values (0.. 255), in cases when overflow may be produced when subtracting the background images, to avoid such problem (clipping), both images grayscale ranges will be adjusted automatically.